6. Two polycarbonate plastic tube caps for plugs.

7. High-purity silicone rubber soft tubing (1/8⁰⁰ ID 1/4⁰⁰ OD:

Nine 5 cm long, thirteen 15 cm long, and three 25 cm long).

8. Tygon^® 3-stop tubing S3™E-LFL 1.85 mm ID.

9. Surgical instruments: three clamps, six forceps, four metal spa-

tulas, two scissors, and one scalpel handle.

10. Stainless-steel instrument drying tray with rolled bead edge.

11. Two worm-drive clamps for firm hose and tube, steel screw,

1/2⁰⁰ wide band, 2–9/16⁰⁰ to 3–1/2⁰⁰ clamp ID.

12. Silicone-based lubricant.

2.3

Other Materials

1. Timing belt (7⁰⁰/35T, (3/8⁰⁰) Wide XL).

2. Small pulley: Timing belt pulley, XL series, 0.929“ OD,

10 teeth with “set-screw” for tightening.

3. Large pulley: Timing belt pulley, XL Series, 1.756“ OD with

“set-screw” for tightening.

4. 37D L shaped gear motor bracket.

Fig. 1 CAD model of the double-chamber tracheal bioreactor. The bioreactor body and lid are shown as

translucent. (a) Top view showing the position of the anchors and the trachea between them with a cable tie

on each end for tracheal anchorage; (b) Lateral view showing the position of the motor, pulleys, belt, and shaft

holder on the bioreactor right side

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